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A goal oriented molecular biologist with a passion for describing effects of small molecules, diet and gut microbiome on host molecular pathways that modulate aging and aging-related diseases.

Scientific Citations

h-Index

i-Index

  • 5 years of post-doctoral research experience: human genetic disorders, microbiome, HIV and aging research.
  • 9+ years of experience in science communication and curation of biomedical resources.
  • Webmaster of The Buck Institute for Research on Aging’s official Blog website. Link
  • Immigration Status: Permanent Resident (USA)

I believe in passion and intensity; there is training for everything else.

  • Ph.D., Molecular and Cellular Biochemistry (2010)
    Friedrich-Alexander University/University of Kentucky
  • M.S., Human Genomics (2004)
    Panjab University, India
  • B.S., Biotechnology (2002)
    Panjab University, India
  • 5 years of post-doctoral research experience: human genetic disorders, microbiome, HIV and aging research.
  • Strong background in cell and molecular biology, biochemical, and cell culture techniques.
  • Proficient in sample preparation and analysis of next generation sequencing (NGS) and microarray.
  • Experience with molecular biology platforms: Bioanalytical assays, Real Time-PCR, Fluorescent Imaging, Flow Cytometry and luminescent plate readers.
  • Experience with human samples to describe genetics of a wide variety of human diseases (AIDS and Prader-Willi syndrome).
  • Experience with generating stable recombinant cell lines for protein expression.
  • Experienced in using different in vivo vertebrate and invertebrate models.
  • Experienced in in situ hybridization, molecular cloning, and cDNA library generation.
  • Experienced with sample preparation and analysis of top-down/bottom-up protein mass spectrometry.
  • Developed a directory of resources for public repositories of genetic, genomic, and biological data.
  • 9+ years of experience in science communication and curation of biomedical resources.
  • Proficient in bioinformatics tools and languages (HTML, dHTML, and CSS).
  • Designed and webmaster of The Buck Institute for Research on Aging’s official Blog website. Link

Positions and Employment

  • Research fellow, Post-Graduate Institute of Medical Education and Research, Chandigarh India (2004-2007)
  • Research Scholar, Unversity of Kentucky, Lexington, KY, USA (2007-2010)
  • Post Doctoral Fellow, Gheens Center on Aging University of Louisville, KY, USA (2010-2011)
  • Post Doctoral Fellow, Buck Institute for Research on Aging, Novato, CA, USA (2011-Present)

Cell Culture

  • Primary neuronal culture
  • Mammalian cell culture. Cell line handles: N2a, HEK 293, NIT1 and HELA cells
  • Macrophage cell culture

 

Molecular Biology: RNA/DNA based techniques

  • Cloning of double-stranded RNAs (small and long RNAs)
  • RNase protection assay (RPA)
  • Next Generation Sequencing (NGS)
  • RNA Pull down assay
  • Northern Blotting
  • Southern Blotting
  • Colony Hybridization
  • Total RNA Isolation: Animal tissues and cells
  • Transient transfection.
  • PCR, Reverse Transcription PCR and Real Time PCR,
  • Minigene cloning
  • Restriction enzyme and bacteriophage lambda based cloning
  • Primer-Designing and Gene Arrays
  • Electrophoresis
  • In-situ hybridization and immunostaining
  • Primary Neuronal Culture

 Proteomics:

  • SDS-PAGE of Proteins,
  • Western Blot, Silver Staining of Proteins
  • Two-Dimensional Gel Electrophoresis

 

Cytogenetics:

  • Preparation and Staining of Metaphysics chromosomes & Barr Body.
  • Immunohistochemistry: Macrophage staining

Radiation Safety Certification from Radiation Safety University of Kentucky Animal Handling Mouse dissection: Mouse tissues and macrophage culture

Bioinformatics: BLAST Alignment of Pair sequences, Multiple Sequence Alignment, Prediction of RNA Secondary Structure Gene and SWISS-PROT, Database Search for Similar Sequences. Developed an online resource: BioInoformatics Support and Analysis (www.bisa.co.in)

Animal Handling Mouse dissection: Mouse tissues extraction, primary neuronal and macrophage culture

Softwares

  • Multifactor Dimensionality Reduction (MDR) software for Gene-Gene and Gene-Environment interactions.
  • MASCOT software for analyzing the MALDI-TOF results
  • PD quest for analyzing the 2D dimensional results
  • ImageJ and Quantity One 3.1 software for studying the DNA/PCR results.
  • Haploview version 3.2 for studying the Linkage disequilibrium for the association between SNPs.
  • PHASE version 2.1 for generating Haplotypes of genes
  • Primer 3 software for designing the primers/oligos.
  • End Note 9 software
  • Khanna A, Kumar J, Misha VA, et al. (2016). Trimethylamine, a human gut flora metabolite, modulates nutrient sensing and lifespan in C. elegans Cell Reports (Under Submission).
  • Khanna A*, Kumar J*, Misha VA*, et al (2016). The influence of dietary bacteria genotypes on dauer formation in C. elegans Cell Metabolism (Under Submission) (*Equal Contributions).
  • Katewa SD, Khanna A, Kapahi P (2014) Mitobolites: the elixir of life. (Cell Metab.) PMC4103626
  • Khanna A, Muthusamy S, Liang R, et al. (2011) Gain of survival signaling by down-regulation of three key miRNAs in brain of calorie-restricted mice. Aging (Albany NY) PMC3091518
  • Liang R*, Khanna A*, Muthusamy S, Li N, Sarojini H, Kopchick JJ, Masternak MM, Bartke A and Wang E. (2011)Post­transcriptional regulation of IGF1Rby key microRNAs inlong­livedmutant mice. (Aging Cell) (*Equal Contributions)
  • Khanna A and Kapahi P. (2011) Rapamycin: Killing two birds with one stone. Aging (Albany NY)
  • Li X, Khanna A, Li N, Wang E. (2011) Circulatory miR34a as an RNA based, noninvasive biomarker for brain aging. (Aging (Albany NY))
  • Shen M, Eyras E, Wu J, Khanna A, Josiah S, Rederstorff M, Zhang MQ, Stamm S. (2011) Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression. (Nucleic Acids Res.)
  • Khanna A and Stamm (2010) S Regulation of alternative splicing by short non-coding nuclear RNAs. (RNA Biol.)
  • Kishore S*, Khanna A*, Zhang Z, Hui J, Balwierz PJ, Stefan M, Beach C, Nicholls RD, Zavolan M, Stamm S. (2010) The snoRNA MBII-52 (SNORD 115) is processed into smaller RNAs and regulates alternative splicing. (Hum Mol Genet.)  *Co-first authors
  • Kishore S, Khanna A, Stamm S. (2008) Rapid generation of splicing reporters with pSpliceExpress. (Gene)
  • Kaul D, Anand PK, Khanna A. (2006) Functional genomics of PPAR-gamma in human immunomodulatory cells. (Mol Cell Biochem.)
  • Kaul D, Khanna A, Suman (2006) Detection of novel miRNA in HIV-1 genome and its role in cd4T-cell deletion. (PINAS India)

Book Chapters

  • Hui J, Kishore S, Khanna A and Stamm S. (2009) Analysis of Alternative splicing with Microarrays. Bioinformatics for Systems Biology, Part III, 267-279, DOI: 10.1007/978-1-59745-440-7_14
  • Zhang Z, Khanna A and Stamm S. (2011) Fast cloning of splicing reporter minigenes. Alternative Pre-mRNA Splicing: Theory and Protocols.

Awards

  • Research assistant fellowship in DBT funded project (2006)
  • Invited as an instructor for the workshop on “Alternative Splicing and Disease” organized by European Alternative Splicing Network (EURASNET) (2009)
  • Thomas C. Vanaman Graduate student best talk award at Spring research conference University of Kentucky (2009)
  • Selected chair to organize Gordon Research seminar on aging and metabolism held in Italy, (2013)

Memberships

  • Student Member of Indian Science Congress (2004-2006)
  • Founder of BioInformatics Support and Analysis (2006- Present  )
  • Webmaster/Member of European Alternative Splicing Network (EURASNET) (2007-2010)

Net Clamor (2014)   NC

Latest scientific discoveries supplemented with comprehensive perceptive.

A universal aspect of science and politics is that those both are exciting, useful and are a global human endeavor.These have a potential to make our lives better. They in themselves are a body of knowledge and an ongoing process! Net Clamor highlights the high impact knowledge that we acquire every day with a sprinkle of fun and thoughtfulness. We pride ourselves in analyzing the content and present a comprehensive picture of the topic at hand.

Doctoral Media (2013)DM

A resource to add a new dimension to your scientific research.
– A platform: Website designing for scientists
– A resource: Scientific Skill Resource Management (SRRM)
– A toolkit: Scientific writing for social media audience
– Consultancy: Training in social media tools tailored for scientists
– Tools for social media “connect.”

Bioinformatics Support and Analysis (2006)BISA

BISA is a  knowledge-based venture with the basic aim of gathering and dissipating bioinformatics based knowledge and aims to provide comprehensive information technology based solutions.

Objectives:
>To assist the scientific community in bioinformatics analysis using available online resources.
>To manage and provide a authenticated protocols to use online resources.
>To support life science researchers in pre/post-wet lab experiments involving data collection and pathway analysis.
>To provide a common platform for science students to discuss bioinformatics based problems and to make them aware of market research and job potentials available in commercial setups.

Email: doctmedia@gmail.com
Ph: +1 859-608-6028

“Have no fear of perfection, you’ll never reach it” – Salvador Dali

Extra Academic Activities

Sequence Support

Sequence Support was conceived as an idea by the name BISA (BioInformatics Support and Analysis) in 2006 and the resource was redesigned as Sequence Support in 2016, is a  knowledge-based venture with the basic aim of gathering and dissipating bioinformatics based knowledge and aims to provide comprehensive information technology based solutions. It has a dedicated student following on social media platforms.

Net Clamor

Latest scientific discoveries supplemented with comprehensive perceptive.

A universal aspect of science and politics is that those both are exciting, useful and are a global human endeavor.These have a potential to make our lives better. They in themselves are a body of knowledge and an ongoing process! Net Clamor highlights the high impact knowledge that we acquire every day with a sprinkle of fun and thoughtfulness. We pride ourselves in analyzing the content and present a comprehensive picture of the topic at hand.

Doctoral Media

A resource to add a new dimension to your scientific research.
– A platform: Website designing for scientists
– A resource: Scientific Skill Resource Management (SRRM)
– A toolkit: Scientific writing for social media audience
– Consultancy: Training in social media tools tailored for scientists
– Tools for social media “connect.”

Mystic Aura: Poems

Life is mystic and everything associated with it has an aura. I wish to share few poems celebrating that aura.